Protein−Protein Interactions: Modeling the Hepatitis C Virus Ion Channel p7
نویسندگان
چکیده
منابع مشابه
Protein-protein interactions: modeling the hepatitis C virus ion channel p7.
The p7 protein is a small ion-channel-forming membrane polypeptide encoded by the hepatitis C virus which consists of two transmembrane alpha-helices, TM1 and TM2, and can be blocked by long-alkyl-chain iminosugar derivatives. The length of TM1 and TM2 was estimated by employing different secondary structure prediction algorithms and is proposed to span from Ala-10 to Leu-32 for TM1 and from Tr...
متن کاملNMR structure and ion channel activity of the p7 protein from hepatitis C virus.
The small membrane protein p7 of hepatitis C virus forms oligomers and exhibits ion channel activity essential for virus infectivity. These viroporin features render p7 an attractive target for antiviral drug development. In this study, p7 from strain HCV-J (genotype 1b) was chemically synthesized and purified for ion channel activity measurements and structure analyses. p7 forms cation-selecti...
متن کاملThe 3-dimensional structure of a hepatitis C virus p7 ion channel by electron microscopy.
Infection with the hepatitis C virus (HCV) has a huge impact on global health putting more than 170 million people at risk of developing severe liver disease. The HCV encoded p7 ion channel is essential for the production of infectious viruses. Despite a growing body of functional data, little is known about the 3-dimensional (3D) structure of the channel. Here, we present the 3D structure of a...
متن کاملTargeting the Hepatitis C virus ion channel p7 for anti-viral therapy
Introduction Hepatitis C virus (HCV) currently infects over 3 % of the world population and is the major indicator for liver transplant surgery in the west. Acute infection is usually asymptomatic but leads to persistence in the majority of cases causing chronic liver disease. Treatment of the virus is currently limited to the use of type 1 interferon either alone, or in combination with the gu...
متن کاملEvidence for the formation of a heptameric ion channel complex by the hepatitis C virus p7 protein in vitro.
The p7 protein of hepatitis C virus functions as an ion channel both in vitro and in cell-based assays and is inhibited by amantadine, long alkyl chain imino-sugar derivatives, and amiloride compounds. Future drug design will be greatly aided by information on the stoichiometry and high resolution structure of p7 ion channel complexes. Here, we have refined a bacterial expression system for p7 ...
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ژورنال
عنوان ژورنال: Journal of Medicinal Chemistry
سال: 2006
ISSN: 0022-2623,1520-4804
DOI: 10.1021/jm050721e